Introduction to the Chromatographic Analysis Course


The course will focus on all aspects of theoretical and practical chromatographic analysis including sample extraction and preparation, analyte separation (chromatography e.g. GC, GCxGC, LC), and mass spectrometric detection. Mass spectrometry is the gold standard in terms of detectors for analytical determinations based on chromatographic separations, as it is the most sensitive and selective detector providing highly accurate and precise data. The course will include instrument configuration selection (quadrupole, tandem, magnetic sector, time-of-flight), analyte ionization and optimization (tuning and calibration).

A pictorial walkthrough of the components of the various instrument configurations will be presented to explain operation and routine maintenance steps as well as their importance to the overall system. Quality assurance and quality control aspects will be emphasised.

Course components include:

  • Sample extraction and preparation considerations to minimize signal suppression and matrix interferences
  • Anatomy of a chromatograph-mass spectrometric system
    • Discussion of key components e.g. chromatograph, ion source, mass analyzer, detector, vacuum system, data system
  • Chromatographic and mass spectrometric theory
  • Chromatographic configurations GC, Fast GC, GCxGC, LC
  • Ionization methods for LC and GC
  • Mass spectrometric scan modes for target and non-target methods
  • Optimization, calibration and typical conditions
  • Visual walkthrough of a quadrupole, time of flight and magnetic sector instrument
  • Routine maintenance and trouble shooting
  • Method Validation, Quality Assurance/Quality Control to Get the Right Answer
  • ISO 17025 – “General requirements for the competence of testing and calibration laboratories”
  • Various applications

Course presenter


Prof Eric Reiner

University of Toronto - Department of Chemistry

Eric Reiner is a adjunct professor at the University of Toronto Department of Chemistry and a visiting scientist at the Laboratory Services Branch of MOECC. He obtained his Ph.D. from the University of Toronto in 1986 in Analytical Chemistry / Mass Spectrometry. Working in the field of ultra-trace environmental analysis for more than 30 years he has co-authored over 180 publications.

His research interests include the analysis of dioxin-like and other emerging toxic persistent and bioaccumulative organics including halogenated flame retardants, perfluorinated compounds and industrial chemicals as well as advanced analytical techniques such as multidimensional chromatography, chemical ionization mass spectrometry, ultrahigh resolution and tandem mass spectrometry and analytical automation.


Registration closes on Monday 23 July 2018.

Delegate Cost: R 2000.00

Full-time Student Cost: R 1750.00

Cost includes VAT, course handouts, light lunch, tea and coffee

Date:     Monday 30 & Tuesday 31 July 2018

Time:     10:00 - 17:00

Venue:   SAGL Auditorium, 477 Witherite Road, The Willows, Pretoria


Map to the course venue

SAGL Auditorium, 477 Witherite Road, The Willows, Pretoria

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Event Organiser - Carlamani Conferences and Events